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The relationship between NAM metabolism and NAD + utilization indicates that elevated NNMT expression may inhibit energy expenditure and promote fat deposition by limiting NAD + -dependent processes (Trammell and Brenner, 2015)

Insight into the mechanism of ferroptosis inhibition by Ferrostatin-1

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Protein thermal shift (Sypro orange) assay To measure thermal stability, 5 pmol of immunopurified FSP1GFP isolated from indicated cell lines or 100 g of recombinant WT or D285N mutant FSP1 were combined with 10 SYPRO orange protein gel stain in a total volume of 25 l in 50 mM sodium phosphate pH 7.4

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