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Accordingly, we found that artificial elevation of glutathione levels may induce SA accumulation in tobacco leaves, either following a single infiltration of exogenous GSH or OTC or continuous exposure to OTC ( Figure 1B , Figures S1 and S2 ) Furthermore, although GSH and OTC treatments of SA-deficient NahG tobacco did not increase free SA levels, bound SA levels were unexpectedly (1.5 to 2-fold) higher in treated NahG plants

Fluorescent and Luminescent Probe-Based Assays Overview Fluorescent probe-based methods have become increasingly popular in glutathione research due to their compatibility with high-throughput screening, live-cell imaging, and microplate reader formats