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Because roGFP2 also is less reducing than roGFP1 we asked whether roGFP2-iX probes can be engineered and whether such probes would have an even less negative redox potential than their respective roGFP1-iX counterparts to enable redox imaging in compartments with a less negative E GSH

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Isolated hepatocytes and livers were subjected to 2 h of in vitro and 30 min of in vivo ischemia, respectively, brief ischemia inducing minimal injury in lean counterparts

The blastocysts were subsequently transferred directly into the TUNEL reaction mixture (at 37 C for 60 min) without undergoing a washing process