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In this study, Nociceptive behavior was quantitatively evaluated using cold allodynia and thermal hyperalgesia assays, histopathological changes in the dorsal horn of the spinal cord were examined using H&E staining, and oxidative stress factors (MDA, SOD, GPX, TAC), inflammation markers (IL1B, TNF-), and liver enzymes (ALT, AST) were measured

The human CBS gene can complement growth defects in cys4 yeast deletion mutants [45, 46], and this assay can also be used to test whether variants are vitamin B 6 -dependent [47,48,49,50]

Key scenarios for separate supplementation include: Diagnosed deficiencies requiring high-dose supplementation

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Fifteen minutes after ligation, venograms were captured and digitised into files on a personal computer and were analysed using ISSA image software (Vamstec, Zagreb, Croatia)

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