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doi: 10.1016/j.metabol.2018.01.005 28 JingXDuTChenKGuoJXiangWYaoXet al

To maintain a stable expression of human FcRn, 250 g/mL Geneticine selective antibiotic (G418 Sulfate) (Gibco) and 5 g/mL Blasticidin S HCl (Gibco) were added to the culture medium

Tsarfaty G, Longo DL, Murphy WJ: Human insulin-like growth factor I exerts hematopoietic growth-promoting effects after in vivo administration

Res 97 , 11151123 (2005)

EBCT = Media Volume (ft) / Flow Rate (ft/min) If flow rate is given in gallons per minute (gpm), convert using: 1 ft = 7.48 gallons Example: Filter media volume: 2,000 ft Flow: 1,500 gpm Flow in ft/min: 1,500 7.48 = 201 ft/min EBCT = 2,000 / 201 = 9.95 min Know your target EBCTtypically 815 minutes for advanced BAC, but check your plant's specs

Notably, the mortality rate during the index admission for ALF was 50%