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Varney MJ, Steyaert W, Coucke PJ, Delanghe JR, Uehling DE, Joseph B, et al

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2020;140(3):574582.e2

Unbiased genome-scale gene perturbation screens have identified a critical role for the transcription factor Ikaros in supporting both RelA positive feedback and TNF production ( The complex relationship between NF-B dynamics and transcriptional control in macrophages was recently investigated further by coupling the dynamics of fluorescently labeled RelA with single cell RNA-seq ( While the amplitude of NF-B translocation to the nucleus can be correlated with inflammatory gene induction and sustained expression, multiple recent studies hint at the importance of the integration of multiple signaling processes ( Tnf transcription by activating NF-B through the canonical pathway ( Tnf promoter activity depends greatly on TRIF's involvement downstream of LPS stimulation ( Tnf (Figure 2)