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We speculate that the peroxynitrite production by misfolded metallated SOD1 may benefit, similarly to the HO formation, by the ability of SOD1 enzyme to accept ubiquitous biological thiols as an alternative substrate for cooper activation

Gambini J, Ingls M, Olaso G, Lopez-Grueso R, Bonet-Costa V, Gimeno-Mallench L, et al

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Buffers containing strong chelators (EDTA, EGTA) or reducing agents (DTT, -mercaptoethanol, TCEP) should be avoided at the reconstitution step, since chelators can strip copper from the GHK-Cu component