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For cell viability assay, 8505 C and SNU-80 cells (1 10 4 and 5 10 3 cells/well) grown in 96-well plates were treated with glutamine-free medium or reagents for the indicated times

The recommended dosage varies based on individual needs

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10.1016/j.ccell.2015.09.021 43 ChiuM.SabinoC.TaurinoG.BianchiM