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bacterial NmABST in green (PDB code: 3ZUX)

Cell Host Microbe 19 , 837848 (2016)

Diabetologia 59 , 560570 (2016)

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The resulting peptides were dissolved in 8 l of 0.1% formic acid, 2% acetonitrile solution, and 2 l of the peptide solution were resolved on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 m, 50 cm, Thermo Scientific), using a 0.1% formic acid, 2% acetonitrile (buffer A) and 0.1% formic acid, 95% acetonitrile (buffer B) gradient at 300 nl/min over a period of 180 min (222% buffer B in 110 min, 2240% in 25 min, 4090% in 5 min, followed by holding at 90% buffer B for 5 min and re-equilibration with buffer A for 25 min)