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Taken together, the data further indicates the complexity of 20S regulation through a variety of mechanisms that coordinate its cellular activity

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These are legitimate reasons to define washouts, but the conversation is different than the receptor needs a rest. Different mechanism, different rationale, different way to think about the timing

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Absorbance was measured at 450 nm using a Thermo Multiskan Sky spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States) and cellular viability was assessed