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Interestingly rats given p53 inhibitors and amifostine did not have improved survival compared to controls, suggesting amifostine acts (at least in part) via a p53 dependent mechanism [55]

Mutational analysis revealed key residues (R63, F64, W65, H71, R72, G73, R80) crucial for enzyme function, despite not directly binding the substrate

Samples were maintained at cryogenic temperatures using a closed cycle liquid He coldhead cryostat (15 K) to minimize radiation damage and maintain an inert sample environment

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This suggests that while ALA can improve semen parameters, whether this translates into higher fertility remains uncertain