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For first-strand cDNA synthesis and for real-time PCR analysis of relative miRNA expression, PerfeCTa universal primer and a PerfeCTa assay primer were used together with PerfeCTa SYBR Green SuperMix (95054

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The disease mechanism centers on disrupted equilibrium between tissue breakdown and rebuilding within the disc, causing deterioration of the extracellular matrix, depletion of proteoglycans and hydration, decreased disc height, and compromised mechanical stability

A weak topical steroid should be instilled at least 4 times a day for 1-2 weeks [5]

doi: 10.1038/s41590-024-01832-x 91 LiaoG.WangY.LiuT

By enhancing pain coping skills and emotional resilience, these interventions contribute to better functional recovery and health-related quality of life (Zhang et al., 2018