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Cells were collected by centrifugation (4,000 g , 30 min), resuspended in lysis buffer (5 ml per gram of cells, 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 5% glycerol and 0.1 mM phenylmethylsulfonyl fluoride) and sonicated for 5 min with 10-s on/off pulses

The premature brain undergoes critical developmental events as neuronal migration, growth of axons and dendrites, synaptogenesis, development of the vascular system, and myelination

Drug-induced : Taking angiotensin-converting enzyme (ACE) inhibitors or non-steroidal anti-inflammatory drugs (NSAIDs)

doi:10.1016/j.lfs.2019.117139 Deng M, Zhang Q, Yan L, et al

Verkade 20 , Andrew Wehrman 21 , Christine Clemson 22 , Qifeng Yu 22 , Quanhong Ni 22 , Jessica Ruvido 22 , Susan Manganaro 22 and Jan P

Take 1 shot (20ml) daily or as directed by healthcare professional