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Superoxide anion radical scavenging assay Although the Superoxide anions radical (O 2 ) is weak oxidant, it eventually generates highly reactive and harmful hydroxyl radicals and singlet oxygen, both of which play a significant role in causing oxidative stress

N epsilon-(carboxymethyl) lysine (CML) as a biomarker of oxidative stress in long-lived tissue proteins

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Collection of lung tissue for immunofluorescent staining Mice were euthanized via intraperitoneal injection of ketamine (130 mg/kg) and xylazine (8.8mg/kg)

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