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While the molecular ruthenium catalyst and the nanoreactors without carbon fluorinated moieties were cytotoxic in the low micromolar range in a 21% O 2 atmosphere, these compounds lost their therapeutic effect in a 1% O 2 atmosphere

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After tipping off the supernatant, plates were washed, blotted on paper, and blocked with PBS 1% BSA for 30 min at RT

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After 1, 5, 10, 15, 20, and 30 min of reduction reaction 5 L of 0.1 mol/L BCPB was added