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To test this prediction empirically, we used two model substrates: leupeptin (N-acetyl-L-leucyl-L-leucyl-L-argininal), a natural tripeptide inhibitor of serine proteases containing a carbonyl group, and oxidized lysozyme as a model protein

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The samples were then sprayed with a layer of gold, and the sperm morphology was observed using the JEOL 7000 field emission gun scanning electron microscope (SEM) (QUANTA 250 FEG, FEI, USA)