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The membranes were incubated overnight at 4 C with MRP2 antibody (ab187644), Nrf2 antibody (ab62352), GPX4 antibody (ab125066), and -actin antibody (ABclonal, AC028) After three washes, the membranes were incubated with secondary antibodies at room temperature for 1 h, washed five times, and visualized using enhanced chemiluminescence reagents

10.1155/2019/9610629 50 ZhouZ.LiangS.ZhouZ.LiuJ.ZhangJ.MengX.et al (2023)

As previously described, we applied MRS on the ACC using a special sequence 37 with the following parameters 20 : TE = 8.5 ms, TR = 3000 ms, average number = 128, and VOI dimensions = 30 20 20 mm 3

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However, long-term exposure to warmth can still cause slow degradation

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