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Given that the degradation of KEAP1 encompasses two distinct pathways, namely autophagy 29 and proteasomal degradation 30 , we employed the protein synthesis inhibitor cycloheximide (CHX), the proteasome inhibitor MG132, and the autophagy inhibitor chloroquine (Chlq) to further explore the underlying mechanism in macrophages (Fig

You, et al

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Statements Data availability statement The data from the single cell transcriptomics have been deposited in the repository GEO of the NCBI accession number GSE 253497 ( The rest of the data presented in the study can be found in the article and Supplementary material

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Notably, the most effective approach for reporter mRNA and gene replacement therapy was the combined use of m 6 A with other nucleoside analogues, which will be discussed below