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Slices were stained with DAPI (F6057, Sigma, USA) and immunofluorescence images observed under a confocal microscope (Leica, DM6000, Wetzlar, Germany)

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and Ciriolo, M.R

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Rats were divided into three groups: sham surgery, transection alone, and transection with recombinant human IGF-1 (rhIGF-1) injection (25 g)