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Furthermore, for patients with ALD, cessation can reduce the synergistic harm caused by combined alcohol and tobacco exposure and may decrease the likelihood of alcohol relapse post-transplant [115]

An aliquot of the cleared lysate was retained to represent the whole cell fraction, and the rest added to pre-washed (in RIPA buffer) streptavidin sepharose beads (Sigma-Aldrich, Cytiva 17511301) for rocking 2 h at 4 C

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* Correspondence: Jun Liu, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Following centrifugation, the resulting pellet was lysed in the specified lysis buffer supplemented with a protease inhibitor cocktail