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Description

Azizi NF, Kumar MR, Yeap SK, Abdullah JO, Khalid M, Omar AR, Osman MA, Mortadza SAS, Alitheen NB (2021) Kefir and Its Biological Activities

10.1016/j.bbagen.2012.09.008 147 LuS

10.1039/d3bm01627g 149 WanY.ZhaoD

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Briefly, Human GrK and its catalytically inactive mutant GrKSA, in which a serine has been replaced with Ala, were produced in Pichia pastoris and purified using cation-exchange chromatography

Cys43 and Cys153 of YADH1 correspond to Arabidopsis GSNOR Cys47 and Cys177 and they are conserved residues in class III alcohol dehydrogenases coordinating the catalytic Zn 2+