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Neurogastroenterol Motil 30(5):e13260

doi:10.1007/s13300-019-00700-4 [11] McGregor B

The reactions were allowed to proceed for 2 h at 37 C and samples were then incubated with anti-HA beads to immunoprecipitated 3xHA-Ub by incubation at 4 C for 8 h