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Nguyen et al

Future research could focus on interdisciplinary breakthroughs (Wang et al., 2022b): applying spatial transcriptomics and single-cell sequencing to map spatial localization of microbiota-ENS interactions, and decipher interaction sites between specific strains and ENS neuron subsets

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The 25-l reaction solution contained 6 g of nuclear extract , 5 l of 5 binding buffer (50 mM Tris-HCl, pH 7.4, with 20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 5 mM DTT, and 0.25 mg/ml poly dI-dC) and 50,000 cpm labeled probe

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