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Changes are quite likely more related to increased systemic inflammation, BBB disruption, and cognitive outcome deterioration (Silva et al., 2020)

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4.6 Relationship between cholinergic system abnormalities and ferroptosis in AD 4.6.1 Relationship between abnormal acetylcholine synthesis/degradation and ferroptosis in AD Cholinergic neurons are widely distributed in the human brain and play crucial roles in neurogenesis, neuronal differentiation, and synaptic plasticity (Long and Holtzman, 2019)

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After incubation, the beads were washed with 1 ml of ice-cold phosphate-buffered saline (PBS), pH 7.4, three times, and the beads were eluted in sample loading buffer by boiling for 10 min at 100 C

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