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doi: 10.1016/j.sjbs.2022.103457 166 SellamiH.KhanS

After centrifugation at 12700 rpm for 25 min at 4 C, 60 l of the supernatant was neutralized with 100 l of 0.25 M CHES-NaOH (pH 9.4), and disulfides were reduced with 35 l of freshly prepared 10 mM dithiothreitol

Microbial mats in the submerged Middle Island Sinkhole of Lake Huron provide opportunities to investigate cyanobacteria under such persistent low-O2 Authors: Voorhies, A.A., B.A