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On the first day, bilateral vertebral arteries were irreversibly electro-cauterized under anaesthesia with sevoflurane (a mixture of 3.5% sevoflurane in 33% O 2 and 66% N 2 O)

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If the phenotypes of the mutants can be successfully restored to wild type, this will confirm that only the correct genes have been deleted and further validate the specific phenotypes ascribed to each GDH mutant

A total number of 2635 raw movie stacks made of 50 frames each were collected with SerialEM 87 from a single individual session with a GLACIOS 200 kV FEG Cryo-TEM (ThermoFisher Scientific) using a K2 Summit detector (Gatan Inc., USA) at 36000 magnification and pixel size of 1.145 /pixel without pre-exposure and using a defocus of 2.6 to 1.8 m and 50 e - / 2 total dose per stack (1 e - / 2 per frame)

In general, pay attention to a conscientious dosage especially in infants, toddlers and older people

doi: 10.3389/fimmu.2019.02090 55 Al-ShaeliSJJHussenTJEthaebAM