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Cycloheximide (Sigma-Aldrich) was added to a final concentration of 100 g/mL in HEK293 and HeLa cells, while, in fibroblasts, they were added to a final concentration of 120 g/mL

A handful of other genes, including ODZ4 , coding for cell surface proteins involved in signaling and neuronal path finding, NCAN , a brain-expressed extracellular matrix glycoprotein (rodents with altered NCAN gene function show manic-like behaviors) and ANK3 , a gene involved in localization of sodium channels, have had replicated GWAS support (20, 33, 34, 37, 38)

Therefore, the development of radiotherapy sensitizers can greatly overcome the shortcomings of conventional radiotherapy and reduce the toxic side effects caused by conventional radiotherapy [12,13,14]

Toxicol Res 37(3):323335

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For clinical translation, non-invasive alternatives (e.g., intranasal NSC-derived exosomes, focused ultrasound-enhanced IV delivery) should be prioritized, as pioneered in recent ASD trials using intranasal routes [37, 38]