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Butyrate exhibits wide-ranging effects, including the regulation of immune function, intestinal hormone production, and lipogenesis

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Under conditions of metabolic stress, such as hypoxia, glucose deprivation, and low serum, ACSS2 enzyme can promote malignant cell growth and survival in some solid tumors through the production of acetyl-CoA, which is further incorporated into metabolic pathways or acts as an epigenetic modulator in the activation of protein acetylation [74]

While in vitro models allow more controlled and complex study designs compared with in vivo models, cultured muscle cell models typically have low endogenous GLUT4 expression [115,116], often lack a mature transverse tubule system [117], and do not recapitulate in vivo insulin responses to the same degree as the 3T3-L1 adipocyte

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