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The cells were incubated with 70% ethanol at 20 C overnight

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Treatment of A549 and HCC827 cells with the food additive ferric ammonium citrate (AFC) for 24 h resulted in a significant decrease in the expression of cell proliferation-related proteins (Ki67, CDK2 and CCND), autophagy-related proteins (ATG3 and LC3A/B) and ferroptosis negative regulators (GPX4 and FTH1), followed by a decrease in intracellular autophagy and induction of cellular ferroptosis [188]

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Wise RA, Holbrook JT, Criner G, Sethi S, Rayapudi S, Sudini KR, Sugar EA, Burke A, Thimmulappa R, Singh A, Talalay P, Fahey JW, Berenson CS, Jacobs MR, Biswal S, Broccoli Sprout Extract Trial Research Group (2016) Lack of effect of oral sulforaphane administration on Nrf2 expression in COPD: a randomized, double-blind, placebo controlled trial