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10.3390/bioengineering8080110 36 LiC.LiL.ChenS.ZhangJ.LW.-L

The C 50 values were then used to calculate apparent DNA binding constants via a derivative of the Cheng-Prusoff equation: K app = (8.8 10 6 M 1 )(12.5/ C 50 ), where 8.8 10 6 M 1 is the binding constant of EtBr, 12.5 is the micromolar concentration of EtBr, and K app is the apparent binding constant of the analyte 21