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Primary outcome measures included safety (determined by maintenance of corneal transparency and absence of rejection, scarring, inflammation or neovascularization detected during clinical examinations up to 6 months post-operatively) and efficacy measures to assess reduction in maximum keratometric power, increase in corneal thickness and improvement in uncorrected and BCVA at 6 months

Alanine scanning mutagenesis revealed several crucial regions that determine the substrate specificity and catalytic activity

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For cell-based systemic regeneration, it is the better-characterized tool [7](#ref-7)[8](#ref-8)

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