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In the second one, called slow, the cells showed low transit velocity in microchannels, which was correlated with membrane transformation according to EMA and Annexin-V flow cytometry tests

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Stimulation of TNFR1 induces certain conformational changes in the receptor, enabling its cytosolic portion to recruit several proteins to form the prosurvival complex I consisting of TNFR-associated death domain (TRADD), TNFR-associated factor 2 and 5 (TRAF2/5), RIPK1, cellular inhibitor of apoptosis proteins 1/2 (cIAP1/2), and linear ubiquitin chain assembly complex (LUBAC) (Seifert and Miller, 2017