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Beppu flasks consist of two rooms separated by a membrane filter that allows medium components, including pectin and its degradation products, to pass through and be maintained at the same level, but does not allow yeast cells to be mixed

found Escin-activated ROS to upregulate p38 expression in a dose- and time-dependent manner and induced apoptosis and autophagy in human osteosarcoma cells, but had a minimal impact on JNK and ERK-2 [28]