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For immunostaining of MBP and neurofilament-200 (NF-200), after fixation of the cells with 4% PFA, the cultures were treated with 0.3% Triton X-100 in PBS for 30 min and incubated with primary antibodies (Table 2) in PBS containing 1% HS overnight at 4 C

Supplementary material The Supplementary Material for this article can be found online at: References 1 BrayFFerlayJSoerjomataramISiegelRLTorreLAJemalA

Vitamin B12 Shots are now offered at Flex Foot and Ankle PPLC

Diabetes Care 17(10):11001109

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