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Description

The dissociated single cells were seeded on AggreWell TM 800 plates (Stemcell Technologies, USA #34850) containing 300 microwells per well, pretreated with AggreWell rinsing solution (Stemcell Technologies, USA #07010), according to the users manual

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Oral NAC at 600-1800 mg daily is well-absorbed and documented to raise intracellular glutathione levels over weeks of use

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Treatments were administered with or without 300 ng/ml Doxycycline, 5 M A, 350 M BSO, 5 M DMF, and 0.1 M Fer-1, as indicated in the culture medium

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