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The cell apoptosis was detected by using a Flow cytometry (Beckman, Navios 2 L 8 C, USA)

R.JohanssonP.JaarsmaT

Additionally, bovine mammary alveolar cells-large T antigen (MAC-T) cell line was used as positive control cells to validate the cytokeratin-18 staining (1:50/1:100)

Veronese N, Stubbs B, Solmi M, et al

Tentative inferences about glutamatergic tone can be made from the metabolic hyperactivity documented in this circuit in OCD

These results suggest that the abundance of free iron contributes to the induction of ferroptosis via increased iron intake and decreased iron storage