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The culture media were then replaced with 150 l of treatment at two final concentrations of 5 and 60 M

Measure and photograph target zones

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It has been postulated that brewed unfiltered coffee contains much more antioxidant substances than ready-to-drink coffee used in our study as well as paper-filtered coffee, 23 possibly resulting in a larger content of antioxidant substances that were absorbed into the systemic circulation following oral administration, and hence a higher total antioxidant status in the plasma

Limitations and Future Directions While our study establishes GPR68 as a central ferroptosis regulator, several questions remain