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Our results indicate that MeCbl may bind to GSDME and thereby inhibit its activity via adopting base-off conformation in coordination to the Cys residue of GSDME

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Acknowledgements This work was partly supported by Associazione Italiana Ricerca sul Cancro (AIRC) (IG2014-15197 to L.F.) and by grants from the University of Chieti-Pescara G

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In the electron transport chain (ETC), FMN and FAD function as cofactors that shuttle electrons, acting especially in I and II complexes, which contain flavoproteins reductases and electron transferring flavoproteins, thereby facilitating the production of adenosine triphosphate (ATP), the primary energy source of cells, being particularly important in the nervous system, due to its high energy demands (43, 45)