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For immunoblotting, protein lysates were prepared in RIPA buffer and separated using NuPAGE Novex 412% Bis-Tris gels, followed by transfer onto a nitrocellulose membrane with the iBlot2 Gel Transfer Device

In this study, childhood allergy risk was greatly influenced by gender and other environmental factors, such as household smoking, maternal ethnicity, and maternal asthma status

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