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In ERR-knockout mice, FAO is impaired, leading to reduced mitochondrial density and contractile dysfunction in the heart

3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work

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Easy bruising, bleeding gums, heavy menstrual cycles, fatigue, brain fog, anxiety, and muscle pains are common symptoms

Retatrutide appears in clinical-trial literature for obesity and metabolic disease (Phase III as of 2025-2026)