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Advertisement Research ArticleImmunology Open Access | 10.1172/JCI170848 1 Department of Internal Medicine and Radboud Center for Infectious Diseases and 2 Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands

Once the cells were 80% confluent, OCSCs were treated with 0.6mM sulfasalazine while BCSCs cells were treated with 0.3mM sulfasalazine for 24 hours

However, this complex surgical procedure is associated with significant postoperative morbidity and mortality

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We screened candidate mutant lines by PCR and Sanger sequencing and identified the Atg8a 12 allele that carries an 8-bp deletion based on sequencing data (Fig S2A), which causes frameshift in all isoforms of the Atg8a gene