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This outcome is consistent with research work done by Jyothi et al

(Relaxing can be particularly hard the first time, but we promise it gets easier!) Rotate your injection sites to avoid bruising, pain, and scarring for example, one butt cheek one day, the other butt cheek the next

This is where affinity tags come in they are peptides which do not naturally exist in most expression systems that can be added to the N or C terminus of your protein of interest

Then, 50 L standard was added to the standard well, 50 L sample dilution buffer to the blank well, and 10 L sample with 40 L dilution buffer to the sample well

Mingorance C, et al