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In brief, a pET-His 6 -MBP-Asn 10 -TEV (1C) expression vector (gift from Dr Scott Gradia, UC Berkeley, via Addgene

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional and/or National Research Committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards

doi: 10.1111/j.1600-065X.2008.00652.x 65 KimPTDemetrisAJOlearyJG