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GlycoBlue (Invitrogen, AM9516) was added to a final 25 g ml 1 to facilitate precipitation and visualization of RNA pellets

Randomized, phase I/II study of gemcitabine plus IGF-1R antagonist (MK-0646) versus gemcitabine plus erlotinib with and without MK-0646 for advanced pancreatic adenocarcinoma

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It is a cofactor in hundreds of enzymes, including those involved with energy production in the mitochondria

HRP-conjugated secondary antibody (Cell Signaling, MA, USA) was then added for 2 h, and the membrane was washed with PBS