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The following conditions were used: 40 cycles at 95 C for 30 s, 60 C for 30 s (annealing), and 72 C for 30 s (extension) 24

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ApInAPDB ( is a recently established database composed of about 850 apoptosis-inducing peptides and their analogues provided from previous literature, including peptides binding target or binding affinity, function, and their effectiveness reported as IC50

This is thought to occur due to berberine's bright yellow pigmentation and its excretion through the gastrointestinal tract, though this mechanism is based primarily on anecdotal reports rather than formal studies