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Membranes were blocked with TBS blocking buffer (Thermo Fisher Scientific 37535) for 1 h at room temperature and incubated with primary antibody diluted in TBS with 0.1% Tween20 (TBS-T): blocking buffer 1:1 overnight at 4 C

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Antioxid Redox Signal 9:18991909

Progressive VEGFAinduced RPE and photoreceptor degeneration and decline in visual function are mediated in part by NLRP3 inflammasomedependent IL1 activation and can be inhibited by targeting Nlrp3, caspase1/caspase11, or Il1r1 VEGFA hyper mice develop progressive basal laminar subRPE deposits and degeneration of the RPE and photoreceptors, resembling aspects of nonexudative AMD (Fig 9B and Appendix Figs S2 and S3)