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Sensitivity was evaluated using samples spiked with a series of concentrations of the positive control antibody (0.24729 ng/mL), and each sample was analyzed independently three times

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After trypsinization to remove surface-bound EVs, recipient cells were collected, and the internalized Halo-Rac1 was quantified by western blotting

2000 Jun 15;151(12):1182-8

Doubling a sermorelin dose does not proportionally increase GH output due to receptor saturation kinetics at the pituitary somatotroph level