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To examine transcriptional changes in vivo, we injected MG3 cells in mice that were then treated with the four conditions (control diet + DMSO local delivery, CMD diet + DMSO local delivery, control diet + RSL3 local delivery and CMD diet + RSL3 local delivery), and harvested the tumors after 3 days of treatment

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Notably, muscle creatine levels showed a consistent inverse correlation with the severity of myalgia, suggesting that creatine depletion may contribute to impaired ATP buffering, mitochondrial dysfunction, and symptom burden

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These double mutants were purified and tested against four non-native substrates