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Materials and Methods Chemicals and Reagents Mercuric chloride (HgCl 2 , 99.5% purity) was purchased from Sigma-Aldrich Chemical Company India and other required chemicals of analytical grade were procured, Merck India and HIMEDIA Labs, India

These designs allow for pre-specified modifications, such as dose adjustments, reallocation of participants, or sample size recalibration, based on interim data, without compromising statistical rigor