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To test the role of the mitoses on the upwards growing layers, we kept the dissected follicle intact and suppressed the mitotic activity of the cells using demecolcine (colcemid) at 0.25 g/mL

TMAO is mainly derived from gut, where the gut microbiota converts TMA precursors into TMA, which is absorbed into the bloodstream through the intestinal mucosa, and then transformed into TMAO by hepatic flavin monooxygenases (FMOs) in the liver

Therefore, this study aimed to verify whether SHBG may restore depleted PPAR functions and thus serve as a new candidate for the management of metabolic conditions

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